Recombinant human Growth hormone receptor protein (Fc Chimera Active) (ab180056) (2024)

• Applications

  Functional Studies

  SDS-PAGE

• Form

  Lyophilized

• Concentration information loading...

General Info

• Alternative names

  • GH receptor
  • GH-binding protein
  • GHBP

  see all

• Function

  Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.
  The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
  Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.

• Tissue specificity

  Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 is expressed in lung, stomach and muscle. Low levels in liver.

• Involvement in disease

  Defects in GHR are a cause of Laron syndrome (LARS) [MIM:262500]. A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone.
  Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA) [MIM:604271]. Short stature is defined by a subnormal rate of growth.

• Sequence similarities

  Belongs to the type I cytokine receptor family. Type 1 subfamily.
  Contains 1 fibronectin type-III domain.

• Domain

  The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  The box 1 motif is required for JAK interaction and/or activation.
  The extracellular domain is the ligand-binding domain representing the growth hormone-binding protein (GHBP).
  The ubiquitination-dependent endocytosis motif (UbE) is required for recruitment of the ubiquitin conjugation system on to the receptor and for its internalization.

• Post-translational
  modifications

  The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17.
  On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2.
  On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization.

• Cellular localization

  Secreted; Cell membrane. On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway and Cell membrane. Remains fixed to the cell membrane and is not internalized.

• Information by UniProt

• SDS-PAGE - Recombinant human Growth hormone receptor protein (Fc Chimera Active) (ab180056)

  Human GHR, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

• Functional Studies - Recombinant human Growth hormone receptor protein (Fc Chimera Active) (ab180056)

  Immobilized Human GH, Tag Free at 2µg/mL (100 µL/well) can bind Human GHR, Fc Tag with a linear range of 0.19-3.1 ng/mL.

• SDS-PAGE - Recombinant human Growth hormone receptor protein (Fc Chimera Active) (ab180056)

  SDS-PAGE analysis of ab180056in reducing (lane 1) and non-reducing (lane 2) conditions. Gel stained overnight with Coomassie Blue. As a result of glycosylation, DTT-reduced protein migrates as 66-85 kDa and non-reduced protein migrates as 135-160 kDa.

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

• Datasheet download

  Download

ab180056 has not yet been referenced specifically in any publications.